![]() (ZIP 2497 kb)Įukaryotic parasites are directly responsible for over one million human deaths yearly, causing widespread infectious diseases, such as malaria or infant diarrheas, and many of the neglected tropical diseases. The PrimerTree results were restricted only to the targeted group to show the diversity of on-target taxonomic groups amplifiable. The figures show, for each primer set, the PrimerTree plot and amplicon lengths. PrimerTree results for each newly designed primer. Overview of the pipeline for the in silico assessment of the primer amplification range, information content and specificity. The percent within each sector is shown in parentheses. The taxa amplified by each of the three primer sets are presented as Venn diagrams showing the overlap in species coverage. Apicomplexa and Nematoda each required three primer pairs to capture the diversity within these groups. Complementarity of the primer pairs targeting the same taxonomic groups. Explanation of each column is presented in the excel sheet. ![]() Green text represents on-target species and red text shows off-target (often host) species amplification. Each column shows the percentage of the reads that match each observed species. The table shows the results of the sequencing assay when performed on pools of DNA from known parasites, with and without addition of Anopheles/Escherichia/Human DNA. Primer characteristics of primers from the literature. Examples of genera targeted by each primer pair.
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